FastQCFastQC Report
Wed 8 May 2024
EGAF00002542605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85824871
Total Bases686.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGCGC3214755637.457156212911755No Hit
GAGGATCT2036745323.731411143047335No Hit
CTTATGAG1828931321.310038438624627No Hit
AGACCATA939812610.950352608161799No Hit
NCCTGCGC6118630.7129203841156952No Hit
NAGGATCT3751920.4371599929349151No Hit
NTTATGAG3391910.395212944741857No Hit
GCCTGCGC3070490.3577622621768928No Hit
GAGGAGCT2986490.3479748894699766No Hit
CGTATGAG2713660.3161857359505964No Hit
TCCGGCGC2520600.2936910910125341No Hit
CCCTGCGC2505600.2919433458862991No Hit
GAGGATCC2324560.27084922737605976No Hit
GAGGAACT2013290.234581185679848No Hit
GAGGATCG2012730.23451593652846853No Hit
TCCAGCGC1900260.2214113435719583No Hit
NGACCATA1755050.20449200558658576No Hit
AGACCAGA1526830.17790064607262854No Hit
TCCCGCGC1424460.1659728681677832No Hit
GAGGATCA1341360.15629036016844117No Hit
CTGATGAG1294330.1508105966159856No Hit
ACCTGCGC1271800.14818548343638058No Hit
GAGGACCT1162530.13545374277346714No Hit
AGACCAAA1005080.11710824476508679No Hit
AGACCACA858440.10002228841101317No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)