FastQCFastQC Report
Wed 8 May 2024
EGAF00002542610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83651865
Total Bases669.2 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGCGG2154501825.755574009019405No Hit
AAGCAGTC1931499223.089732667645844No Hit
TGCACAAA1924685023.00827363502296No Hit
GCAGTTCT1773771121.204202679760936No Hit
AAGCAGGC4972930.5944792743114573No Hit
NTTTGCGG4131300.4938682478866431No Hit
NGCACAAA3805800.45495698153292813No Hit
NAGCAGTC3704480.4428448785929638No Hit
NCAGTTCT3343540.39969700615760334No Hit
GCAGGTCT3047900.36435529560518465No Hit
GGCACAAA3011990.3600625042848716No Hit
AAGCAGCC2677120.3200311194496381No Hit
AAGCAGAC2288980.27363167575522673No Hit
CGTTGCGG2112780.25256818840799306No Hit
GCAGATCT1675300.20027049008411227No Hit
CTTGGCGG1674270.20014736073128794No Hit
CGCACAAA1564400.18701316461982048No Hit
AGCACAAA1542720.18442147105745937No Hit
GCAGCTCT1503190.1796959338563462No Hit
CTGTGCGG1405470.1680141859359621No Hit
GCAGTGCT1399240.16726943266596625No Hit
GCAGTTCG1069590.12786206260912414No Hit
CTATGCGG919250.10988995881920864No Hit
CCTTGCGG886650.10599285503078743No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)