FastQCFastQC Report
Wed 8 May 2024
EGAF00002542611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84028622
Total Bases672.2 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGCGG2162046725.72988403879811No Hit
AAGCAGTC1957824823.29949906830556No Hit
TGCACAAA1939580823.08238257197649No Hit
GCAGTTCT1782885921.217602497396662No Hit
AAGCAGGC4815790.5731130518836784No Hit
NTTTGCGG4036850.48041368570818643No Hit
NGCACAAA3718850.44256943782798197No Hit
NAGCAGTC3649810.43435318979763826No Hit
NCAGTTCT3265760.388648525022819No Hit
GCAGGTCT3050080.3629810804228112No Hit
GGCACAAA2865640.3410314166522926No Hit
AAGCAGCC2583600.3074666629663402No Hit
AAGCAGAC2131760.2536945089971843No Hit
CGTTGCGG2127630.2532030098030169No Hit
CTTGGCGG1865370.22199221593804075No Hit
GCAGATCT1634080.1944670709939763No Hit
CGCACAAA1505290.17914015060249353No Hit
AGCACAAA1487610.1770361056260092No Hit
GCAGCTCT1465530.1744084295467799No Hit
GCAGTGCT1400240.16663845802445743No Hit
CTGTGCGG1215250.14462334036609575No Hit
GCAGTTCG1080030.12853120452219247No Hit
CCTTGCGG872010.10377535406923606No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)