FastQCFastQC Report
Wed 8 May 2024
EGAF00002542616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86869979
Total Bases694.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGCTG2389077527.501762145009845No Hit
TCCAATGC2085644424.00880515925991No Hit
GTAGCACA1926967822.182206352323398No Hit
CGTTTGAT1692741919.48592505127692No Hit
AAGCGCGG6513580.7498079399788965No Hit
NAGCGCTG4575830.5267446881735749No Hit
AAGCGCCG4231550.48711304511769254No Hit
NCCAATGC4111840.4733326803267674No Hit
NTAGCACA3698280.42572590008338784No Hit
GCCAATGC3697830.4256740985283305No Hit
NGTTTGAT3228720.37167270409953707No Hit
AAGCGCAG2616490.3011961128711681No Hit
CGGTTGAT2493670.28705774177751325No Hit
GGAGCACA2268120.26109365123709766No Hit
CGTTTGAG1704110.19616788441954155No Hit
GCAGCACA1561130.1797088036593171No Hit
ACCAATGC1232820.14191554023513692No Hit
GAAGCACA1061120.12215036911658514No Hit
CGATTGAT1050950.12097965397228885No Hit
CGCTTGAT1036730.11934272483247636No Hit
TCCAAGGC995240.11456662145618798No Hit
TCCAACGC989200.11387132947275144No Hit
CCCAATGC930500.10711410440193614No Hit
CGTGTGAT916030.10544839662042511No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)