FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002542622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96687304
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT2637900327.282799197710588No Hit
CTATAGTG2548515226.358323115514732Clontech Universal Primer Mix Short (100% over 8bp)
AGTCCCAC1891947219.567690086797747No Hit
TACGTTCA1796048818.575849420726428No Hit
GCGAGAGG11702761.2103719429388577No Hit
GCGAGAGC6866440.7101697654120132No Hit
GCGAGAGA5814260.6013467910947232No Hit
NCGAGAGT4959840.5129773811875031No Hit
NTATAGTG4873680.5040661801884558No Hit
CTATAGGG4816400.4981419277136944Clontech Universal Primer Mix Short (100% over 8bp)
NGTCCCAC3611090.3734813000887893No Hit
NACGTTCA3431710.35492870915089325No Hit
AGGCCCAC3368420.34838286524154194No Hit
TACGGTCA2960100.3061518811197797No Hit
AGCCCCAC2127360.22002475112968298No Hit
CGATAGTG2036960.21067502306197305No Hit
AGACCCAC1940360.20068405258253968No Hit
CTAGAGTG1704270.1762661621012827No Hit
TACGATCA1620360.16758767004197364No Hit
TACGTGCA1532630.15851408991608662No Hit
TACGCTCA1494540.15457458613180486No Hit
CTATAGAG1176060.1216354114083065No Hit
CTATAGCG1167470.12074698038948319No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)