FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002542623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96775813
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT2643194027.312547609390787No Hit
CTATAGTG2561030526.463538983650803Clontech Universal Primer Mix Short (100% over 8bp)
AGTCCCAC1897769419.60995564046566No Hit
TACGTTCA1805556418.657103919137317No Hit
GCGAGAGG11659901.2048361712032325No Hit
GCGAGAGC6817580.7044714778061332No Hit
GCGAGAGA5732570.5923556539897009No Hit
NCGAGAGT4818090.49786096862859736No Hit
NTATAGTG4745830.49039422691287543No Hit
CTATAGGG4514140.4664533275478657Clontech Universal Primer Mix Short (100% over 8bp)
NGTCCCAC3539390.36573084640477266No Hit
NACGTTCA3359650.34715802387524247No Hit
AGGCCCAC3163150.3268533636601947No Hit
TACGGTCA2970870.3069847628146508No Hit
CGATAGTG2068160.2137062904343671No Hit
AGCCCCAC1946600.20114530063415742No Hit
CTAGAGTG1912800.1976526924139609No Hit
AGACCCAC1712570.17696260531544178No Hit
TACGATCA1584230.16370102723910984No Hit
TACGTGCA1568640.16209008753044524No Hit
TACGCTCA1477380.15266004533591465No Hit
CTATAGCG1011660.1045364506521893No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)