FastQCFastQC Report
Wed 8 May 2024
EGAF00002542628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103232538
Total Bases825.8 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGAGC3290753131.877091891318223No Hit
GCGGATAG2393993523.1902997483216No Hit
TTATCGTT2205157121.361066411057337No Hit
CATCTCCA1788806117.327929107003065No Hit
NGCAGAGC6242940.6047453759201387No Hit
NCGGATAG4516940.4375500290421998No Hit
NTATCGTT4164210.4033815384835351No Hit
GCGGAGAG3806880.3687674519830172No Hit
TTATCGGT3656920.3542410242786049No Hit
GTATCGTT3371170.3265607981080539No Hit
NATCTCCA3313180.320943383180214No Hit
CAGCTCCA2952860.28603965931749153No Hit
CAACTCCA2444300.23677612188513666No Hit
CATCCCCA2419660.23438927753573197No Hit
GCGGAAAG2399420.23242865539157817No Hit
CATCGCCA2178230.21100227139625297No Hit
GCGGACAG1946190.18852486219025247No Hit
TTATCGCT1416920.1372551743327283No Hit
CACCTCCA1315650.12744528280414844No Hit
TTATCGAT1268270.12285564460306111No Hit
TTATCGTG1088360.10542799984245277No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)