FastQCFastQC Report
Wed 8 May 2024
EGAF00002542629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102930794
Total Bases823.4 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGAGC3276795231.834935617032155No Hit
GCGGATAG2381042523.13246024314162No Hit
TTATCGTT2177584521.15581173890488No Hit
CATCTCCA1778327217.276921034923717No Hit
NGCAGAGC6466010.6281900438852147No Hit
NCGGATAG4631680.4499800127841237No Hit
NTATCGTT4117080.3999852561129568No Hit
GCGGAGAG4043940.39287951086824413No Hit
TTATCGGT4024050.39094714454451795No Hit
GTATCGTT3976970.38637319750977533No Hit
NATCTCCA3312770.3218444035319498No Hit
CAGCTCCA3075490.2987920213653457No Hit
GCGGAAAG2418010.2349160932344503No Hit
CATCCCCA2400600.23322466549709117No Hit
CAACTCCA2367230.22998268137327302No Hit
GCGGACAG2061670.20029671586911107No Hit
CATCGCCA1975620.1919367298381085No Hit
TTATCGCT1479490.14373638271944156No Hit
TTATCGTG1476330.14342938032713515No Hit
CACCTCCA1379060.1339793414981332No Hit
TTATCGAT1349790.13113568326306702No Hit
ANCAGAGC1041260.10116117437119936No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)