FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002542634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117148946
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCGCGC3725581331.802089794303402No Hit
GGCGAGTA2610925122.287226553451024No Hit
ACTTCTAT2425518120.704566134124672No Hit
CAAATACG2085922617.805730834317536No Hit
GGCGAGGA9449200.8065970990468834No Hit
NTGCGCGC7212980.615710191707572No Hit
GGCGAGCA6588030.5623635743167505No Hit
GTGCGCGC6479470.5530967389156024No Hit
GGCGAGAA4984760.4255061757021698No Hit
NGCGAGTA4797860.40955212691371545No Hit
NCTTCTAT4499860.38411442472559676No Hit
NAAATACG3915670.33424713868104283No Hit
CAAAAACG3660160.3124364430901495No Hit
CAAAGACG3502750.2989997024813181No Hit
TGGCGCGC2656690.226778822235413No Hit
TCGCGCGC2027660.17308393026429789No Hit
CTGCGCGC1872370.1598281558589524No Hit
CAAACACG1817340.15513071709582432No Hit
ACTGCTAT1513490.1291936506197845No Hit
ACCTCTAT1456510.12432975709401602No Hit
ACGTCTAT1329970.11352812341990683No Hit
ACATCTAT1249770.10668213779746683No Hit
ACTTCTAG1196420.10212810621445967No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)