FastQCFastQC Report
Fri 10 May 2024
EGAF00002542635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116961886
Total Bases935.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCGCGC3702197631.653025841255673No Hit
GGCGAGTA2597506522.208144796844334No Hit
ACTTCTAT2405243020.564331529332556No Hit
CAAATACG2075042017.74118108868388No Hit
GGCGAGGA10129110.8660180120556537No Hit
GTGCGCGC7470170.6386841265538417No Hit
NTGCGCGC7440890.6361807469486256No Hit
GGCGAGCA6608110.5649797746934416No Hit
GGCGAGAA5134540.43899257917232964No Hit
NGCGAGTA4772630.4080500206708363No Hit
NCTTCTAT4449460.3804196522617633No Hit
NAAATACG4015130.3432853331383524No Hit
CAAAAACG3607620.3084440686943095No Hit
CAAAGACG3327930.28453115060063244No Hit
TGGCGCGC2778840.23758508818847193No Hit
TCGCGCGC2042480.17462782705128405No Hit
CTGCGCGC1971960.16859851251030614No Hit
CAAACACG1771110.15142625179624752No Hit
ACTGCTAT1646900.1408065529996669No Hit
ACTTCTAG1630270.13938472230175905No Hit
ACCTCTAT1540820.13173693180699908No Hit
ACGTCTAT1425080.12184140053965956No Hit
ATGCGCGC1329550.11367378258589297No Hit
ACATCTAT1234800.10557285302324895No Hit
TNGCGCGC1201050.10268729763813829No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)