FastQCFastQC Report
Fri 10 May 2024
EGAF00002542640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129420060
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATGGG3435554526.54576500737212No Hit
GTCTCCTT3300904125.505351334252204No Hit
AGTGGAAC3040733223.495068693369483No Hit
TCACATCA2270549517.544030654907747Illumina PCR Primer Index 1 (100% over 8bp)
CAGAGGGG7007210.5414315215121983No Hit
NAGATGGG6498010.5020867707834473No Hit
NTCTCCTT6031640.46605139883260754No Hit
NGTGGAAC5751490.4444048318320977No Hit
AGGGGAAC5236650.4046242908556834No Hit
GGCTCCTT5026770.388407330362851No Hit
NCACATCA4361710.3370196243148087No Hit
AGAGGAAC3685340.2847580197382075No Hit
CAGACGGG3619890.27970084390317856No Hit
AGCGGAAC3229380.24952700531895908No Hit
GACTCCTT2965840.22916385605137257No Hit
GCACATCA2874220.2220845825600761No Hit
GCCTCCTT2766230.21374043560171432No Hit
TCACAGCA2600280.20091784843864235No Hit
GTCTCCGT2444550.18888493793002414No Hit
CAGAAGGG2173250.16792219073302855No Hit
TCACACCA1418900.1096352451080613No Hit
GTCCCCTT1332240.10293921977783042No Hit
GTCTCCTG1308330.10109174729172588No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)