FastQCFastQC Report
Fri 10 May 2024
EGAF00002542641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129183920
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATGGG3429609526.548269320206412No Hit
GTCTCCTT3272454425.33174717101014No Hit
AGTGGAAC3026576723.4284321144613No Hit
TCACATCA2252211717.434148925036492Illumina PCR Primer Index 1 (100% over 8bp)
NAGATGGG6711220.5195089295943334No Hit
CAGAGGGG6626380.5129415487624156No Hit
NGTGGAAC5867870.454226036800865No Hit
NTCTCCTT5865290.45402632154218575No Hit
AGGGGAAC5477280.4239908496351559No Hit
GGCTCCTT5411740.4189174627925829No Hit
NCACATCA4463760.3455352647605058No Hit
AGAGGAAC3620540.28026243513898635No Hit
CAGACGGG3441420.2663969323736267No Hit
GCACATCA3373400.26113157117387364No Hit
AGCGGAAC3351440.2594316692046502No Hit
GACTCCTT2973630.2301857692505383No Hit
TCACAGCA2839390.21979438307801777No Hit
GTCTCCGT2805170.2171454465849929No Hit
GCCTCCTT2696150.20870631577056958No Hit
CAGAAGGG1962790.1519376405360667No Hit
GTCTCCTG1926010.14909053696466248No Hit
TCACACCA1535910.11889328021629937No Hit
GTCCCCTT1446870.11200078152141536No Hit
GTCGCCTT1352600.1047034336781234No Hit
CAGATGGA1338610.10362048155838591No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)