FastQCFastQC Report
Wed 8 May 2024
EGAF00002542646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113797871
Total Bases910.3 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCGC3498314630.741476701264475No Hit
GGCTAGCG2832846724.89367046242895No Hit
TCTCGTTT2679781123.548604876799498No Hit
CAAGTAAA1606787614.119663099848326No Hit
NTGACCGC6757650.5938292114445621No Hit
NGCTAGCG5443660.4783622006425761No Hit
GGCAAGCG5292540.4650825145929136No Hit
NCTCGTTT4938680.433987029511299No Hit
GGCCAGCG4435810.3897972748541139No Hit
GGCGAGCG4383370.38518910428473657No Hit
TCTCGGTT3357830.2950696678675122No Hit
NAAGTAAA3089960.2715305631684445No Hit
AGGACCGC2980910.26194778283681597No Hit
TCTCGTGT2919570.25655752382221636No Hit
CAAGGAAA2846770.25016021609051015No Hit
TCTCGTTG2201850.19348780259693962No Hit
ACGACCGC1994870.17529941311467945No Hit
CAAGAAAA1591010.13981017272282711No Hit
TCCCGTTT1497370.1315815477778139No Hit
CAAGCAAA1427380.12543116909454308No Hit
TCTCGTTA1367190.12014196645207889No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)