FastQCFastQC Report
Wed 8 May 2024
EGAF00002542647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113141438
Total Bases905.1 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCGC3477847630.738937576522584No Hit
GGCTAGCG2807161824.811084688529412No Hit
TCTCGTTT2686951223.748603937666058No Hit
CAAGTAAA1596214214.108130745165179No Hit
NTGACCGC7679250.6787301041727966No Hit
NGCTAGCG6216560.5494503260600241No Hit
NCTCGTTT5851630.5171960073549711No Hit
GGCAAGCG5388110.476227816726176No Hit
GGCCAGCG4370170.38625724378719667No Hit
GGCGAGCG3810090.33675460267704926No Hit
NAAGTAAA3526270.3116691870223534No Hit
TCTCGGTT3069560.27130289788256007No Hit
AGGACCGC2865680.25328297489024315No Hit
CAAGGAAA2683480.23717923754866896No Hit
TCTCGTGT2410780.21307666250450166No Hit
TCTCGTTG2035760.17993053968431974No Hit
ACGACCGC1863030.16466380779074064No Hit
CAAGAAAA1512930.133720237849549No Hit
TCCCGTTT1476300.13048269724130604No Hit
CAAGCAAA1313640.11610600176391607No Hit
TCTCGTTA1192300.105381372296152No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)