FastQCFastQC Report
Fri 10 May 2024
EGAF00002542653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117494040
Total Bases939.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACCGT3055190226.00293768092407No Hit
CTTGGTAC2802417523.851571535032758No Hit
AAGTTGCA2612498022.235153374588194No Hit
GCACAATG2511151621.372587069097293No Hit
NGCACCGT6786720.5776224904684527No Hit
TGCACCGG6268800.5335419566813773No Hit
NTTGGTAC6195440.5272982357232758No Hit
NAGTTGCA5778360.4918002649325872No Hit
NCACAATG5555080.47279674781801695No Hit
GCACAAGG5082830.4326032197037399No Hit
GCACAACG3360650.28602727423450586No Hit
GCACAAAG3115690.2651785571421325No Hit
TGCACCGA2770220.2357753635843997No Hit
CGTGGTAC2595800.22093035527589316No Hit
TGCACCGC2592810.22067587428264446No Hit
GGCACCGT1945320.1655675470857926No Hit
CTTGGGAC1873580.15946170546182598No Hit
CCTGGTAC1781120.15159237013213606No Hit
CTGGGTAC1564890.1331888834531522No Hit
AAGTGGCA1510100.1285256681955953No Hit
AAGGTGCA1222050.10400953103663813No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)