FastQCFastQC Report
Fri 10 May 2024
EGAF00002542658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120468839
Total Bases963.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGCTC3770254131.296508966937086No Hit
CGTCCTAG2525033820.960057563101444No Hit
GAGTTAGT2476868920.560245458993755No Hit
TTAAAGCA2337424619.402732021016654No Hit
ACCGGCCC10171980.8443660688055606No Hit
ACCGGCGC9317760.7734581056268003No Hit
NCCGGCTC7156530.5940565261029868No Hit
GAGGTAGT5157060.42808248529729753No Hit
NGTCCTAG4800530.3984872801837162No Hit
ACCGGCAC4663610.387121685467559No Hit
NTAAAGCA4600020.38184314202613007No Hit
NAGTTAGT4589720.38098814914286677No Hit
GAGTTAGG4235060.3515481708925575No Hit
CGGCCTAG3649710.3029588423276828No Hit
CGCCCTAG2996720.24875478379931928No Hit
GTAAAGCA2896980.24047546436468933No Hit
GAGATAGT2869080.23815951276827696No Hit
CGACCTAG2322410.1927809730116184No Hit
TGAAAGCA2252680.18699275420094322No Hit
GAGTTAGA1764370.14645862072265842No Hit
GAGCTAGT1567860.13014651863624252No Hit
ATAAAGCA1366700.11344842461709123No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)