FastQCFastQC Report
Fri 10 May 2024
EGAF00002542659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002542659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119590056
Total Bases956.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGCTC3745355031.31828117882979No Hit
CGTCCTAG2503834020.936807655646554No Hit
GAGTTAGT2473000720.678982707391658No Hit
TTAAAGCA2321524719.412355656058892No Hit
ACCGGCCC10221580.8547182217223814No Hit
ACCGGCGC8574120.7169592762796264No Hit
NCCGGCTC8218150.687193423506717No Hit
NGTCCTAG5522870.4618168253052745No Hit
NAGTTAGT5387210.4504730727778905No Hit
NTAAAGCA5232010.4374954051363602No Hit
GAGGTAGT4682220.3915225192301942No Hit
ACCGGCAC4212050.35220737750971537No Hit
GAGTTAGG3971660.3321062078940744No Hit
CGGCCTAG3529530.29513574272429477No Hit
GTAAAGCA2905640.2429666894712383No Hit
CGCCCTAG2826690.23636497001055No Hit
GAGATAGT2453130.20512825915893876No Hit
CGACCTAG2268560.18969470170663688No Hit
TGAAAGCA2122170.17745371738934548No Hit
GAGTTAGA1491130.12468678833965927No Hit
ATAAAGCA1332160.1113938770962696No Hit
GAGCTAGT1318300.11023491785972572No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)