FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002543751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002543751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374833787
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCCTT12375337333.01553309547306No Hit
CAGATGGG9256732224.695565130578796No Hit
AGTGGAAC8063305021.511681389596824No Hit
TCACATCA6536060917.437224515729156Illumina PCR Primer Index 1 (100% over 8bp)
NTCTCCTT16182190.4317164183494483No Hit
NAGATGGG11994560.3199967669936862No Hit
NGTGGAAC10506910.2803085091152682No Hit
GGCTCCTT8938760.2384726326711845No Hit
NCACATCA8470270.22597402618883983No Hit
GTCTCCTA3908270.1042667479706145No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)