FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002544519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002544519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398083598
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACGCT12954456732.542050878469No Hit
AAGGGTGA10526375126.442624496174293No Hit
CGTTAATC7925257519.908525595671488No Hit
TTACTCAG7279544318.28647132555308No Hit
AAGGGGGA15325470.3849811968389614No Hit
NCCACGCT7621260.19144873183144812No Hit
NAGGGTGA6336500.15917510874185778No Hit
NGTTAATC4873200.12241649805426046No Hit
GCCACGCG4554990.11442295093002047No Hit
NTACTCAG4495780.11293557490404316No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)