FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002546129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54753030
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5404650.9870960566017991Illumina Single End PCR Primer 1 (100% over 50bp)
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG698220.1275217097574326No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA653430.11934134056142645No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT625410.11422381555870058No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG559810.10224274346095549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)