FastQCFastQC Report
Thu 23 Mar 2023
EGAF00002546140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60597060
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT6203611.023747686768962No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT5720470.9440177460754697No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA5588120.9221767524695091No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG5199330.8580168740859704No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC5102070.841966590458349No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT4672460.7710704116668367No Hit
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG4582060.7561521961626522No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA4030780.6651774855083729No Hit
GTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGAC3666230.6050178011936553No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA3439020.5675225827787684No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG3254390.5370541079055651No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG2958990.4883058683045019No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2919950.48186331152039386No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC2587910.4270685739539179No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA2488590.4106783398402497No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT2436190.402031055632072No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2109230.34807464256516735No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC2101780.3468452099821345No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1679940.27723127161614775No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1657680.27355782607275003No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT1532250.252858802060694No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA1511890.24949890308209674No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1454730.24006610221684022No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG1402460.2314402711946751No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT1289060.21272649201132862No Hit
TGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGG1219770.20129194386658364No Hit
CCCCACTATGCACATTTTATTTCTCCAACATACTCGGATTCTACCCTAGC1182440.19513157899079592No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1140430.18819889941855264No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG1110150.183201957322682No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC1065610.1758517657457309No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC1061200.17512400766637853No Hit
CACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCAC1046500.17269814740187064No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1010080.16668795482817153No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT998220.16473076416578628No Hit
CCCCTATCTAGGCCTTCTTACGAGCCAAAACCTGCCCCTACTCCTCCTAG977830.16136591445195525No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT976330.16111837769027076No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG964460.15915953678280761No Hit
CCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACAC901410.14875474156667007No Hit
GGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGA862630.14235509115458736No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC852900.14074940269379407No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC841670.13889617747131627No Hit
CCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG840880.13876580811016245No Hit
CTCCAAATCACCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGC803680.13262689642038739No Hit
CCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCT769690.12701771340061713No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT685070.11305333955145679No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC684920.11302858587528834No Hit
ACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACT673820.1111968138388232No Hit
CTCCAACATACTCGGATTCTACCCTAGCATCACACACCGCACAATCCCCT637990.10528398572471998No Hit
CGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCA634210.10466019308527509No Hit
ATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTG619150.10217492399796295No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT616900.10180361885543622No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA609110.10051807793975483No Hit
CCTCATTCCATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATA608580.10043061495062633No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)