FastQCFastQC Report
Wed 22 Mar 2023
EGAF00002546147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53608478
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC28761635.365127135301249TruSeq Adapter, Index 7 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC8070821.505511870715673TruSeq Adapter, Index 7 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGCC745800.13911978623978094TruSeq Adapter, Index 7 (98% over 50bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA558940.10426335923955908No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)