FastQCFastQC Report
Thu 23 Mar 2023
EGAF00002546164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58287891
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC18275083.1353133020372965TruSeq Adapter, Index 2 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC3570850.6126229545687285TruSeq Adapter, Index 2 (100% over 50bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT819250.14055234902906336No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA648660.11128554985803141No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT647450.11107795957139709No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT622710.10683351023971685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)