FastQCFastQC Report
Tue 19 Sep 2023
EGAF00002546179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39938579
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC23667365.925939428140396TruSeq Adapter, Index 8 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC2883320.7219385546992044TruSeq Adapter, Index 8 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGCC528100.13222803946029227TruSeq Adapter, Index 8 (98% over 50bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA483430.12104336511321546No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGT452900.11339912719478577TruSeq Adapter, Index 8 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)