FastQCFastQC Report
Sat 13 May 2023
EGAF00002546226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30302044
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG3337801.101509851942661No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2289470.7555496916313632No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2000050.6600379829162679No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1943800.6414748787243527No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1825710.6025039102972723No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1760910.581119214268186No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1692600.5585761805375241No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1533360.5060252701104916No Hit
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG1505880.4969565749426012No Hit
GTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGAC1489110.4914222948128516No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1432290.47267108449845824No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1387170.4577809998559833No Hit
CCGCTTTCCACACAGACATCATAACAAAAAATTTTCACCAAACCCCCCCC1284930.4240407016767582No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1149560.3793671476419215No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1119100.36931502046528614No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1033770.34115520391957715No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1002740.33091497062046377No Hit
GGACATAATAATAATAATTGAATGTCTGCACAGCCGCTTTCCACACAGAC777900.25671535557139313No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC775780.25601573280007117No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC732130.24161076394714495No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG724590.23912248295857533No Hit
GCCGCTTTCCACACAGACATCATAACAAAAAATTTTCACCAAACCCCCCC719510.23744602839333215No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC671940.2217474174349427TruSeq Adapter, Index 2 (100% over 50bp)
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT642950.21218040604785607No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT625490.20641841850668557No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT615890.20325031539126537No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT572940.18907635405717185No Hit
GTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTTCACCA551570.18202402451795No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC518490.1711072691993979No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT499910.16497566962809504No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC499100.16470836092773147No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG495320.16346092032603476No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG484870.1600123080806034No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA446430.14732669518927502No Hit
ATTTATCGCACCTACGTTCAATATTACAGACGAACATACTTACTAAAGTG413790.13655514459684634No Hit
CCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACAC413550.13647594201896082No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT404430.13346624405931165No Hit
TAATAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAA397180.13107366618568703No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC396580.13087565974097326No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA393720.12993182902117098No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG380650.12561858863382286No Hit
CTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTTCACCAAA363450.11994240388536166No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC353360.11661259550675857No Hit
TATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC351140.11587997166131764No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT349050.11519024921223137No Hit
TGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGG325590.10744819722392325No Hit
CCCCACTATGCACATTTTATTTCTCCAACATACTCGGATTCTACCCTAGC318370.1050655196725343No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT313560.10347816800741232No Hit
CATAATAATAATAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATC309460.10212512396853492No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT307360.10143210141203675No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA304020.10032986553646349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)