FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002546255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70662793
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8125351.1498767109304608Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1226350.17354960764146415Illumina Single End PCR Primer 1 (100% over 50bp)
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG792590.11216511071109232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)