FastQCFastQC Report
Tue 4 Apr 2023
EGAF00002546268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42894282
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2075280.4838127375578871Illumina Single End PCR Primer 1 (100% over 50bp)
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG868820.20254914163151164No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG662280.15439820160645187No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG474890.11071172609906374No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)