FastQCFastQC Report
Sun 14 May 2023
EGAF00002546291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33504918
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2660210.7939759768998689No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1975590.5896417952731596No Hit
CCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCC1789230.5340201101223409No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1726300.5152377928517837No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1666370.49735086652055077No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1499100.44742685238029833No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC1423110.4247465998872165No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1334090.39817736608100335No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1283080.3829527354760277No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1215030.3626422843356907No Hit
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG1141730.34076489905153623No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1140270.34032914212773185No Hit
GTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGAC1139780.34018289494097553No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC985110.29401952274588466No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC964100.2877488015341509TruSeq Adapter, Index 3 (100% over 50bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA947280.2827286430010066No Hit
GCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCC925480.27622213550858415No Hit
GGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGAC851750.25421641085645996No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG835930.24949471596975703No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA798200.23823368258952313No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC644860.1924672670441993No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC615640.18374615929518168No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG615010.1835581271979236No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT541540.16163000309387415No Hit
CATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATC518900.15487278613844094No Hit
TAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAA514670.1536102849139938No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT491230.14661429704140747No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG484630.14464443697489424No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT479590.14314018019682961No Hit
GTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCA461990.13788722001946102No Hit
ATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTG456170.13615016159717208No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC450030.1343175948080219No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT444720.13273275284541808No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT438900.13099569442312917No Hit
CCCCACTATGCACATTTTATTTCTCCAACATACTCGGATTCTACCCTAGC436870.13038981322085313No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC417220.12452500256827968No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG405140.1209195617192676No Hit
GTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACA395100.11792298670899597No Hit
GGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACAT386360.11531441443909816No Hit
CCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACAC352720.10527409737280957No Hit
CTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCAC349060.10418172042683405No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC344130.10271029464987798No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT335270.10006590674240719No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)