FastQCFastQC Report
Mon 27 Mar 2023
EGAF00002546320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52187331
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1647350.31566090245159306No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1604790.3075056664614636No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1396370.2675687706658154No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1141220.21867759437630563No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1000910.19179175880828242No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC883800.16935144661833731No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC836280.16024578838875664TruSeq Adapter, Index 8 (100% over 50bp)
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA796720.15266540455958552No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT672770.12891442944265535No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA565250.10831172799390719No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)