FastQCFastQC Report
Wed 27 Sep 2023
EGAF00002546377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44325873
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG5772671.302325167966799No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT3673090.8286559860874032No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA3322750.7496186256726404No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC3210700.724339935730087No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3198770.721648505377435No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC3156250.71205591370981No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG3124410.7048727500527739No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT3121330.70417789628193No Hit
GTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGAC3112530.7021925997938044No Hit
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG2497860.5635218961169699No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2451740.5531171377042027No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2415530.5449480938593132No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2330920.5258599193297332No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG2304650.519933358108931No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC2233550.5038930648923712No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA2127470.4799612181355119No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1875200.4230486334696668TruSeq Adapter, Index 2 (100% over 50bp)
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC1698690.3832276467515936No Hit
CCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCCC1434880.32371161646382013No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG1299590.29318993897762596No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1276540.28798981578997895No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT1174690.265012264958662No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT1148570.2591195440189074No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1104400.24915470925975897No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC1063890.24001557735817183No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT1008870.22760296226991403No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC930860.21000376010642813No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC917910.20708221584265243No Hit
CCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACAC908060.20486003738719372No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG894720.20185050839269425No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG882580.1991117016465756No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA860660.19416650857615372No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG833390.18801434548170096No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT833140.1879579450132883No Hit
GGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGAC796990.1798024372808179No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC774580.17474669929230724No Hit
GCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCAAACCCCCCC773480.17449853723129152No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT737530.16638814987355127No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA734860.1657857928709041No Hit
TGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGG716100.1615535017212182No Hit
TATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC696850.15721066565344352No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA669530.15104722246530825No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT646440.14583807520271513No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT632970.1427992179646411No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG605970.13670796737607402No Hit
TAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATCATAACAAA600270.1354220366962654No Hit
CCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCT595040.1342421388970726No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC566850.12788242207886125No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT543430.12259882619796343No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT506390.114242532797944No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT487710.11002828979815016No Hit
GTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC475530.10728045897708546No Hit
GTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCA473540.1068315112485207No Hit
GGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGA464860.1048732869852332No Hit
CATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCACACAGACATC464450.10478079021703644No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA455070.10266464464219352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)