FastQCFastQC Report
Fri 24 Mar 2023
EGAF00002546380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51947301
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1474280.28380300258525465Illumina Single End PCR Primer 1 (100% over 50bp)
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG983620.18934958719029502No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG787890.1516710175183115No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG567980.10933773055889853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)