FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002546523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36027282
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2189350.6076922483355808TruSeq Adapter, Index 5 (100% over 50bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1255640.34852476520432485No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT814030.2259482133567556No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG755580.20972439719432623No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA652070.1809933927294321No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG650520.18056316321614269No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA650200.18047434163920553No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC643610.17864517228915575No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG518390.14388817896393072No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC501110.13909181380932373TruSeq Adapter, Index 5 (100% over 50bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC433690.12037821781837443No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT425720.1181660054177831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)