FastQCFastQC Report
Sun 14 May 2023
EGAF00002546574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25953260
Sequences flagged as poor quality0
Sequence length150
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT2143790.8260195443655247No Hit
CACCATATTGATGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACT1690580.651394083055462No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1639870.6318551118433676No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG867540.3342701456387367Illumina Single End PCR Primer 1 (100% over 50bp)
CTCCGTTTCCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCG827930.3190080937808969No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG817360.3149353876931068No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC787620.3034763262881041No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG739950.28510869154780555No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG725120.2793945731673015No Hit
GTGGCTATTCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGA699450.26950371552552554No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC698900.2692917960980624No Hit
CCCTCCTTAGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCG645990.24890514717611584No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT624590.24065955490755303No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT606670.23375483465275654No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT576540.22214550310828002No Hit
CCCTCCTTAGGCAACCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGA551440.21247427105496575No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT515070.19846061727890832No Hit
CTCGCTATGTTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTC501900.1933861102612928No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA490790.18910533782653893No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT486880.18759878335130153No Hit
CCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC459540.17706446126613767No Hit
GTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAG442580.17052963673927668No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT416900.16063492601700133No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA407650.15707082655512256No Hit
CCCCTCCTTAGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTC404050.15568371757536434No Hit
CCTCCTTAGGCAACCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG399800.15404615836314978No Hit
CCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAA380820.14673301157542443No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG377270.14536516799816285No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT352460.13580567527932907No Hit
CCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC350660.13511212078944995No Hit
CCTCCTTAGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGG346470.13349768006023136No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT345900.13327805447176963No Hit
GTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAG339140.13067337205422364No Hit
ACCATATTGATGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTC338130.13028421092379144No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT328770.12667772757642007No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT318440.12269749542061381No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG279190.10757415446074983No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT268080.10329338202599596No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC266330.10261909293861349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)