FastQCFastQC Report
Wed 6 Sep 2023
EGAF00002546624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38046891
Sequences flagged as poor quality0
Sequence length150
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT2751260.7231234741361653No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT2085340.5480973465085491No Hit
CACCATATTGATGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACT1402250.3685583665692947No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC988550.2598241207146203No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG983750.25856251960245585No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG947030.24891127109439767No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC919680.2417227730907106No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG879620.23119366047543807No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT872150.2292302937446321No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT810350.2129871794255147No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG772380.20300738896116372Illumina Single End PCR Primer 1 (100% over 50bp)
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA736590.19360057566858746No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT735830.19340082215916146No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT668430.17568583987585215No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT631440.16596362630523476No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA628190.1651094172188734No Hit
CCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTC620150.16299623535599794No Hit
GTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAG613650.16128781718327523No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT606820.15949266393409123No Hit
CTCCGTTTCCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCG604160.15879352665110008No Hit
CCCTCCTTAGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCG553190.14539689984130372No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG535110.14064486898548426No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT503030.1322131682191851No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT491260.12911961715873185No Hit
CCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAA490690.1289698020266623No Hit
GTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAG484270.12728241053914235No Hit
CTCGCTATGTTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTC481250.12648865317273886No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT476860.12533481382223846No Hit
GTGGCTATTCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGA474970.12483805838432371No Hit
CCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTC466440.12259608807458144No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT438160.11516315485541251No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT432570.11369391522687096No Hit
CCCTCCTTAGGCAACCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGA424030.11144931658147836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)