FastQCFastQC Report
Wed 27 Sep 2023
EGAF00002546752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002546752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31243464
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT1457240.46641435149444377No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1244640.3983681194889273Illumina Single End PCR Primer 1 (100% over 50bp)
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1176550.3765747613644889No Hit
CACCATATTGATGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACT1069330.3422571837745008No Hit
GTGGCTATTCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGA960220.3073346796629209No Hit
CTCCGTTTCCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCG681380.2180872133768522No Hit
CTCGCTATGTTGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTC552390.17680177844556544No Hit
CCCTCCTTAGGCAACCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGA459220.14698114139968604No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG452210.1447374721317713No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG444300.1422057426154795No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT443250.14186967232570627No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG439400.1406374145965377No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC432660.1384801634031361No Hit
CCCTCCTTAGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCG420780.13467776812455878No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC413590.13237648680696865No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT408520.13075374740777782No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA406530.13011681419192186No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT386670.12376028471106788No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT368450.11792866501614545No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA367290.11755738736268168No Hit
CTCCGTTTCCGACCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACT342560.10964213187116513No Hit
CCTCCTTAGGCAACCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG336310.1076417134796577No Hit
GTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAG334890.10718721842110722No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT334640.10710720168544692No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG316440.10128198332937731No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT314410.1006322474358157No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)