FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002553660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002553660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68068869
Sequences flagged as poor quality0
Sequence length8
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NGAACACG1590203723.361688292485074No Hit
AGAACACG1212254717.809238170241965No Hit
AGATCTCG45639476.704896183892815Illumina Single End PCR Primer 1 (100% over 8bp)
NGATCTCG38087665.595459504402813No Hit
AGATCACG32811764.820376845103743No Hit
NGATCACG31795494.671076582747394No Hit
AGAACTCG21186623.1125271083907684No Hit
NGAACTCG18394552.702344003982202No Hit
NGAACAAG14873902.1851251854941207No Hit
NGAACACA12066361.7726693828275595No Hit
NGAAAACG11992581.761830360366352No Hit
NGAAAAAG10019961.4720326850149368No Hit
AGAACAAG9953021.4621985272004445No Hit
AGAAAACG9133791.341845418351229No Hit
AGAACACA8377371.2307197288675387No Hit
NAAAAAAA7147081.0499777805915946No Hit
AAAAAAAA5764790.846905506833087No Hit
NGAAAAAA5690010.8359195743358099No Hit
AGAAAAAG4854530.7131791774004648No Hit
NGAACAAA4639870.6816434690577862No Hit
AAAACACG4202540.6173953029835122No Hit
NAAACACG3668330.5389144926148252No Hit
NAAACACA3629920.5332716781293957No Hit
AGAAAAAA3305450.4856037787259254No Hit
AGAACAAA3268910.48023568600794586No Hit
NGAAAACA3029450.44505660877074366No Hit
AAAACACA2993450.43976784747224174No Hit
NAAACAAA2784610.40908715553948755No Hit
AAAACAAA2514350.36938324919134474No Hit
AGACCACG2496700.36679028705471806No Hit
AGAAAACA2470200.36289717109887637No Hit
AGAGCACG2300080.33790483576273317No Hit
NAAAAAAG2127390.3125349416338914No Hit
AAAAAAAG1853910.27235798496960484No Hit
NAAACAAG1756120.25799165254236855No Hit
AAAACAAG1729420.2540691545793129No Hit
NGAGCACG1716500.252171076913295No Hit
AAAAAACA1656860.24340936236211005No Hit
AGAACCCG1504990.22109813518423524No Hit
NAAAAACA1419760.20857699281003186No Hit
AAAAAACG1361580.2000297669115084No Hit
NGACCACG1057160.15530741373123153No Hit
NAAAAACG977460.14359868385649247No Hit
AGATCCCG856120.12577262007981946No Hit
AGACCTCG782920.11501880543953212No Hit
NACAAAAA777470.11421814574295336No Hit
AGAGCTCG771080.11327939061246926Illumina Single End Adapter 1 (100% over 8bp)
AGATCGCG765850.11251105112382578No Hit
AGACCCCG753760.11073490878774554No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)