FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002553678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002553678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97565311
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCGCGC3147118632.25653224228435No Hit
GGCGAGTA2502500625.649491344316015No Hit
ACTTCTAT2090237621.42398336638316No Hit
CAAATACG1592810016.32557702808942No Hit
CAAATACA3576270.36655138628113426No Hit
TTTCGCGC3218770.32990926457457814No Hit
AATTCTAT2342560.24010173041932903No Hit
CAAATACT2129410.21825482624659495No Hit
NTGCGCGC1997010.20468442928450256No Hit
NGCGAGTA1587680.1627299686463358No Hit
NCTTCTAT1341710.13751916395777183No Hit
GACGAGTA1301680.13341627128109088No Hit
TTACGCGC1243850.12748895967748208No Hit
GGCGAGAA1096670.11240368003336759No Hit
TTGCTCGC1012390.10376536390070033No Hit
NAAATACG1006040.10311451782283561No Hit
AGCGAGTA994470.10192864552033253No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)