FastQCFastQC Report
Mon 12 Dec 2022
EGAF00002554369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002554369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274069933
Sequences flagged as poor quality0
Sequence length250
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC73522282.6826102081033456TruSeq Adapter, Index 1 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC19092220.6966185524626666TruSeq Adapter, Index 1 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAACTCGTATGCC9652500.3521911321808511TruSeq Adapter, Index 1 (98% over 50bp)
CGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTG7697740.28086773020811445No Hit
CGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACT7619210.2780024031311746No Hit
CGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAA7222310.263520697835906No Hit
CGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTACGCCGATG7111700.25948486658695247No Hit
CGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGTATA7002880.2555143471356269No Hit
CGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT6947520.2534944247240722No Hit
CGGGTCCATCATCCACAACCTTAACAATGAACAAGATATTCGAAAAATAG6926770.25273731869011695No Hit
CGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA6639710.24226334962471055No Hit
CGGCGCAGTCATTCTCATAATCGCCCACGGACTTACATCCTCATTACTAT6072530.22156863153609774No Hit
CGGCTGCCAGGCGTTTAATGGGGTTTAGTAGGGTGGGGTTATTTTCGTTA5908880.21559752780324135No Hit
CGGAGATGAAAACCTTTTTCCAAGGACAAATCAGAGAAAAAGTCTTTAAC5545990.20235674666290374No Hit
CGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATAT5485340.20014380782148766No Hit
CGGTTAGCGGGGGCAGGCCTCCTAGGGAGAGGAGGGTGGATGGAATTAAG5300890.19341377370278703No Hit
CGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATA4064090.14828660537527844No Hit
CGGCAGGTTTGAAGCTGCTTCTTCGAATTTGCAATTCAATATGAAAATCA4035220.14723322459454172No Hit
CGGAGCGAGGAGAGTAGCACTCTTGTGCGGGATATTGATTTCACGGAGGA3977460.14512573329231265No Hit
CGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGA3871900.14127416158415304No Hit
CGGTGGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTG3643610.1329445357291345No Hit
CGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATAT3459140.12621377186967825No Hit
CGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAG3159210.1152702146280307No Hit
CGGCGGCGGGAGAAGTAGATTGAAGCCAGTTGATTAGGGTGCTTAGCTGT3014380.10998579694621227No Hit
CGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAA2927700.10682310051135745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)