FastQCFastQC Report
Wed 16 Nov 2022
EGAF00002554370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002554370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274069933
Sequences flagged as poor quality0
Sequence length250
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG75785482.765187672009246Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT7916570.288852188685725Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAA7508750.2739720449378881No Hit
CGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACT7419250.270706455056491No Hit
CGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTG7263720.26503162607041614No Hit
CGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGTATA6817730.24875877209047953No Hit
CGGCGCAGTCATTCTCATAATCGCCCACGGACTTACATCCTCATTACTAT6788290.24768459369820767No Hit
CGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTACGCCGATG6784870.24755980802899671No Hit
CGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA6641900.2423432562374509No Hit
CGGCTGCCAGGCGTTTAATGGGGTTTAGTAGGGTGGGGTTATTTTCGTTA6286730.2293841550287824No Hit
CGGGTCCATCATCCACAACCTTAACAATGAACAAGATATTCGAAAAATAG6155750.2246050828202304No Hit
CGGTTAGCGGGGGCAGGCCTCCTAGGGAGAGGAGGGTGGATGGAATTAAG5870600.21420080399698568No Hit
CGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT5734230.20922506665479426No Hit
CGGAGCGAGGAGAGTAGCACTCTTGTGCGGGATATTGATTTCACGGAGGA4986330.18193641109840386No Hit
CGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTCAATCATAT4361200.1591272691703836No Hit
CGGAGATGAAAACCTTTTTCCAAGGACAAATCAGAGAAAAAGTCTTTAAC3844140.1402612814153532No Hit
CGGCGGCGGGAGAAGTAGATTGAAGCCAGTTGATTAGGGTGCTTAGCTGT3831900.1398146800729141No Hit
CGGGCTCTGCCATCTTAACAAACCCTGTTCTTGGGTGGGTGTGGGTATAA3788710.1382388049111538No Hit
CGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATAT3595750.1311982661009371No Hit
CGGTGGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTG3471840.1266771572494966No Hit
CGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAG3199990.11675815602873883No Hit
CGGCAGGTTTGAAGCTGCTTCTTCGAATTTGCAATTCAATATGAAAATCA3199530.11674137199135959No Hit
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGT3074140.11216626232400327Illumina Single End PCR Primer 1 (98% over 50bp)
CGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGA2958040.1079301172376322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)