FastQCFastQC Report
Mon 7 Nov 2022
EGAF00002554371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002554371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268527192
Sequences flagged as poor quality0
Sequence length250
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC13407820.4993095820254955TruSeq Adapter, Index 6 (100% over 50bp)
CGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACT7759020.2889472735409232No Hit
CGGGTCCATCATCCACAACCTTAACAATGAACAAGATATTCGAAAAATAG7671110.28567348963303496No Hit
CGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTACGCCGATG7482700.27865706799630185No Hit
CGGTGGGGATAGCGATGATTATGGTAGCGGAGGTGAAATATGCTCGTGTG7445610.2772758298533878No Hit
CGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGTC7197350.26803058365873056No Hit
CGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGTATA6961610.25925158447268165No Hit
CGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCTATCTCAATCATAT6919280.257675207805398No Hit
CGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA6833240.2544710630273898No Hit
CGGCTGCCAGGCGTTTAATGGGGTTTAGTAGGGTGGGGTTATTTTCGTTA6438760.23978055823858613No Hit
CGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTG6319890.23535381846915526No Hit
CGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAAAAAACAATA6226900.23189085446512248No Hit
CGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAA6216740.2315124942728333No Hit
CGGAGATGAAAACCTTTTTCCAAGGGCAAATCAGAGAAAAAGTCTTTAAC5700140.2122742191412779No Hit
CGGCGCAGTCATTCTCATAATCGCCCACGGACTTACATCCTCATTACTAT5597080.20843624656083246No Hit
CGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGT5576120.20765569246335397No Hit
CGGTTAGCGGGGGCAGGCCTCCTAGGGAGAGGAGGGTGGATGGAATTAAG5413500.2015996949761423No Hit
CGGCAGGTTTGAAGCTGCTTCTTCGAATTTGCAATTCAATATGAAAATCA4951720.18440292631518673No Hit
CGGCGGCGGGAGAAGTAGATTGAAGCCAGTTGATTAGGGTGCTTAGCTGT3955050.1472867596962024No Hit
CGGAGCGAGGAGAGTAGCACTCTTGTGCGGGATATTGATTTCACGGAGGA3708480.13810444939967198No Hit
CGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGA3543640.1319657787208381No Hit
CGGCCTCCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAG2970950.11063870209464671No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)