FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002558139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002558139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2660950
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2271398.536011574813507TruSeq Adapter, Index 6 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353341.3278716247956557No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG126420.47509348165129No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT119710.4498769236550856No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA114390.42988406396211876No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG111120.41759521975234404No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG105920.398053326819369No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA101870.38283319866964804No Hit
GCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAG87110.327364287190665No Hit
GCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGT84380.31710479340085307No Hit
GTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTT83650.31436141227757003No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG72210.2713692478250249No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC68690.2581408895319341No Hit
CGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTA63230.23762190195231025No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA60020.22555854112253143No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG59650.22416806027922362No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG59330.22296548225257898No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT57500.21608823916270506No Hit
GTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGC54580.2051147146695729No Hit
TGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTT54250.20387455607959565No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA51260.19263796764313498No Hit
TGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGG51010.19169845355981888No Hit
CTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAG50240.18880475018320525No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC50230.1887671696198726No Hit
CCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTT50000.18790281666322178No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA49370.1855352411732652No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT49340.18542249948326728No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT49170.18478362990661232No Hit
CTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGG48000.1803867039966929No Hit
CTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCA47270.17764332287340986No Hit
GCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGG46430.17448655555346776No Hit
GGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGG45760.1719686578101806No Hit
TTTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGAC45670.17163043274018677No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA42940.16137093895037488No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA42510.15975497472707118No Hit
GTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTG42280.15889062177042035No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA40720.15302805389052782No Hit
GATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTT39880.14987128657058568No Hit
GGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTT38900.14618839136398656No Hit
TTCCTTATGAGCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACT38070.14306920460737704No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT37570.14119017644074486No Hit
GCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCT36510.13720663672748454No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT36400.13679325053082544No Hit
ACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTC36210.1360792198275052No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT36010.13532760856085232No Hit
GCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATA35010.1315695522275879No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG34760.13063003814427177No Hit
GCAGAAGGTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTT34480.12957778237095774No Hit
TAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTT34420.12935229899096187No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC34390.12923955730096395No Hit
GGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAGAAGGTATA33870.12728536800766643No Hit
GCGCCGGGGAGCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCG33390.12548150096769953No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG33370.12540633984103422No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG32980.12394069787106107No Hit
GGCGGGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTA32610.12255021702775323No Hit
TTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTC32330.12149796125443921No Hit
CGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGT32310.12142280012777391No Hit
GGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTAC32260.12123489731111069No Hit
GGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTG32150.12082151111445161No Hit
TGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCG31820.11958135252447433No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG31620.11882974125782145No Hit
GCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTA31450.11819087168116651No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT31300.11762716323117682No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT30210.1135308818279186No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC30100.1131174956312595No Hit
TGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTA29980.1126665288712678No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA29680.11153911197128845No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT29540.11101298408463144No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT29480.11078750070463556No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC29450.11067475901463764No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT29440.11063717845130498No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT29430.11059959788797234No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGC29060.1092091170446645No Hit
GGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAA29000.10898363366466862No Hit
TTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTTA28980.10890847253800336No Hit
GAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGT28560.10733008887803229No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC28290.10631541366805089No Hit
CTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC28170.10586444690805916No Hit
GGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTAC28080.10552622183806536No Hit
CGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGGGTGTTAAATTT27750.10428606324808809No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC27110.10188090719479885No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA26870.10097897367481538No Hit
GTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATT26660.10018978184482985No Hit
GCCGGGGAGCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGG26640.10011462071816456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)