FastQCFastQC Report
Fri 17 Mar 2023
EGAF00002558608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002558608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84604538
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA2974980.3516336204093449No Hit
CCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAA2603860.3077683610777474No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1805140.21336207757555511Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAA1552960.18355516579973524No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1394960.16488004461415534No Hit
GGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGACC1365190.1613613208312774No Hit
GGGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGAC1152690.1362444648063677No Hit
GCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAA1128960.13343965071944486No Hit
CCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCTGAACATCTCCTTCCCA926520.10951185620799678No Hit
CGGGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGA919810.10871875454245727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)