FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002558610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002558610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97558983
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA2253520.23099051780808336No Hit
GTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAA1757870.18018535515073994No Hit
CCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAA1635710.1676636994053126No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1418410.1453899944815948Illumina Single End PCR Primer 1 (100% over 50bp)
GCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAA1226760.12574546825688004No Hit
CCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAAG1113250.1141104556204732No Hit
GGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGACC1000520.1025553946170185No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT977540.10019989650773625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)