FastQCFastQC Report
Tue 14 Feb 2023
EGAF00002558614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002558614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83065835
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5854960.704857779374637Illumina Single End PCR Primer 1 (100% over 50bp)
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA2084220.2509118219301594No Hit
CACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAAGTT2042050.24583512583723502No Hit
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA1996560.2403587467699566No Hit
CTTGACTCCGCTGCTCGCCATGTCTTCTCACAAGACTTTCAGGATTAAGC1797950.2164487963071701No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT1459110.17565705563544867No Hit
CCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAA1458440.17557639672194952No Hit
GCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAAGTTCCA1275660.15357216357362807No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG1183400.1424653107983565No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA963040.11593695530779893No Hit
GCGAGAATGAAGACTATTCTCAGCAATCAGACTGTCGACATTCCAGAAAA939120.11305731170944107No Hit
GAAGACTATTCTCAGCAATCAGACTGTCGACATTCCAGAAAATGTCGACA897040.10799145039594198No Hit
GCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAA870480.10479398660110982No Hit
CCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAAG868210.10452070938671716No Hit
CTGGAGACGACGTGCAGAAATGGCACCTCGAAAGGGGAAGGAAAAGAAGG832080.10017114737966577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)