FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002558628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002558628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92034764
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA2454500.26669270320506283No Hit
CCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAA1939220.21070516354015967No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1850040.20101534676614155No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1774220.19277715538011267Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAA1640640.17826307459211826No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1276050.13864869583410894No Hit
GCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAA1181990.12842864463693304No Hit
GGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGACC1038830.11287365282970682No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC988840.10744200963018714No Hit
CCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAAG921190.10009152628456786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)