FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002558636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002558636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98153017
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2272190.23149466714813258No Hit
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA2249470.2291799140519542No Hit
GTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAA2129300.21693678554985224No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1953880.19906469100180588No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1810270.18443345455188606Illumina Single End PCR Primer 1 (100% over 50bp)
CCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAA1585760.16155998546636627No Hit
GCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAA1532400.15612357590597548No Hit
CCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAAG1352760.1378215404219312No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC1111950.11328739900068481No Hit
GGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGACC989630.10082522476104835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)