FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002558640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002558640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75483242
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA2639620.34969616169904305No Hit
CCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAA2358060.3123951671286191No Hit
GTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAA1876910.24865254197746303No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1427190.18907375494020248No Hit
GCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAA1309780.1735193090937986No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1177040.15593394888894677Illumina Single End PCR Primer 1 (100% over 50bp)
GGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGACC1114830.1476923844897918No Hit
GGGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGAC882540.11691866652998291No Hit
CAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGC862780.11430086693944598No Hit
CCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGAAAG820210.10866120456246434No Hit
GGCCTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGA807910.10703170380519693No Hit
CCTCGGAGGCGTTCAGCTGCTTCAAGATGAAGCTGAACATCTCCTTCCCA800770.10608579848756365No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG759730.1006488301072177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)