FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002637478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002637478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732517
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCTTAGATCTCGTA78411.0704188435217203Illumina Paired End PCR Primer 2 (97% over 36bp)
CCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGG11890.16231705202746147No Hit
GTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCG11290.15412611584441044No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC10240.1397919775240711No Hit
TCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGG9510.129826338501359No Hit
CGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCC8340.11385401294440947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)