FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002637510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002637510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721599
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCAGAATCTCGTA76561.0609770800680156Illumina Paired End PCR Primer 2 (97% over 39bp)
GTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCG12240.16962329493250405No Hit
CCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGG10740.14883612643587366No Hit
CGTGGTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCC9710.1345622707348541No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC9560.1324835538851911No Hit
TCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGG9140.12666314670613457No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)