FastQCFastQC Report
Sun 5 Mar 2023
EGAF00002637722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002637722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59318102
Sequences flagged as poor quality0
Sequence length150-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2831400.47732478021633257No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG2063200.3478196251120779No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1064430.17944437939029134No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC996330.16796390417211932Illumina Single End PCR Primer 1 (96% over 33bp)
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC822180.138605243977631No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA782340.13188891310109685No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT709780.11965655947656585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)